کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1172327 | 1491185 | 2006 | 6 صفحه PDF | دانلود رایگان |
In this work, a new method to control polymerase chain reaction (PCR) thermal cycling in a microchannel using Joule heating effect was presented. Joule heating was generated internally by the current flow through the buffer solution in an electrokinetically-driven microfluidic chip without external heater component. Numerical simulations were developed and conducted to determine the parameters required to achieve the desired thermal cycling. A PDMS-based microchannel PCR chip was fabricated and tested. The power consumption was only 1.3 W for such a Joule heating-controlled PCR chip. To demonstrate the feasibility of using Joule heating to control thermal cycling, a DNA fragment of Escherichia coli O157:H7 stx1 was successfully amplified by a two-temperature TaqMan real-time polymerase chain reaction.
Journal: Analytica Chimica Acta - Volume 557, Issues 1–2, 31 January 2006, Pages 146–151