Extended multiplicative signal correction in vibrational spectroscopy, a tutorial

Extended multiplicative signal correction (EMSC) is a model-based preprocessing technique that has been frequently used in vibrational spectroscopy. The framework of EMSC allows, apart from basic preprocessing as baseline correction and normalization, to separate and quantify different types of chemical and physical variations in vibrational spectra. EMSC thus enables the user to study these different effects separately. The aim of this tutorial is to give a comprehensive description of EMSC and its use in vibrational spectroscopy. Examples from FT-IR and Raman spectroscopy of biological materials will be used, and different extensions of EMSC and their properties will be discussed.