On the generalised case of sequential standard addition calibration

► The generalised case of sequential standard addition calibration has been described. ► An expression for the gradient, systematic bias and relative precision associated with the technique has been derived. ► It has been shown that for single point addition the generalised case is equivalent to previous, non-generalised, considerations. ► The linearity of multi-point addition shows a strong dependence on the solution mass and analyte concentration. ► The findings provide a new tool for analytical chemists to quantify analyte content in unknown solutions.