کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1181478 962945 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nonparametric multivariate regression methods to determine dexamethasone concentration using ELISA measurements
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Nonparametric multivariate regression methods to determine dexamethasone concentration using ELISA measurements
چکیده انگلیسی

Dexamethasone is a synthetic steroid used in veterinary medicine, but its presence in food must remain below certain limits to avoid risk to human health. HPLC methods determine dexamethasone concentration with a high level of accuracy but these methods are expensive and the field instrumentation is difficult to obtain. On the other hand, the classical ELISA method can be used to estimate dexamethasone concentration but it suffers from natural steroid interferences which result in false negatives and positives. In this article we propose the concept of multi-detection in order to estimate dexamethasone concentration from a vector of absorbances. The function which maps the absorbance vector into dexamethasone concentration is calculated by means of non-linear regression algorithms. Results demonstrate that the proposed methods perform much better than the classical ELISA method when interfering steroids are present. This implies that results of immunoassays with cross-reactivity could be treated with nonparametric multivariate regression methods to improve accuracy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemometrics and Intelligent Laboratory Systems - Volume 100, Issue 1, 15 January 2010, Pages 41–47
نویسندگان
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