کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1182133 | 1491631 | 2014 | 6 صفحه PDF | دانلود رایگان |
A dielectrophoresis (DEP)-based microfluidic chip for cell patterning was designed and fabricated to achieve non-contact and batch manipulation of cells. The microfluidic chip employed a polydimethylsiloxane (PDMS) microchannel and two indium tin oxide (ITO) electrodes which were designed as a “step” structure. The distribution of the electric field caused by the microelectrodes was simulated by finite element simulation software COMSOL. The position of the maximum intensity of the electric field was also determined. The ITO microelectrodes and PDMS microchannel were fabricated using the micro-electro-mechanical system (MEMS) technique. After oxygen plasma surface treatment, the PDMS microchannel and glass substrate with the ITO microelectrodes were aligned and bonded to form the experimental microfluidic chip. Through DEP experiments with varying frequencies, the DEP behavior of yeast cells was examined, and the electric field frequency of both positive and negative DEP responses were determined. The results showed that yeast cells with solution conductivity of 60 μS cm−1 exhibited negative DEP movement in a frequency range of 1–10 kHz, positive DEP movement from 100 kHz to 10 MHz, and no DEP movement at 50 kHz. Under a sinusoidal potential of 8Vp-p with frequency of 5 MHz, the yeast cells were aligned into chains along the “step” edge of microelectrodes.
A dielectrophoresis (DEP)-based microfluidic chip was designed to achieve cell patterning. This microfluidic chip employed a polydimethylsiloxane (PDMS) microchannel and two indium tin oxide (ITO) electrodes which were designed as a “step” structure. Under an AC potential of 8 Vp−p with frequency of 5 MHz, the yeast cells were aligned into chains along the step edge of microelectrodes.Figure optionsDownload as PowerPoint slide
Journal: Chinese Journal of Analytical Chemistry - Volume 42, Issue 11, November 2014, Pages 1568–1573