Recent Advances in the Dehydrogenase Biosensors Based on Carbon Nanotube Modified Electrodes

Nicotinamide adenine dinucleotide (NADH) is involved as a cofactor in over 300 enzymatic reaction of NAD+/NADH dependent dehydrogenases. The application of amperometric NADH sensors has evolved to provide a promising measurement technique for detection of substrate or enzymatic activity. However, the direct oxidation of NADH at ordinary electrodes often requires high overpotential and suffers from low sensitivity and the fouling of the electrode surface by its oxidation products. In recent years, carbon nanotubes (CNT) are attracting growing attention in decreasing the high overpotential for NADH oxidation and minimizing the surface fouling. This paper introduces the research progress of the NADH electrochemical sensors based on CNT-modified electrodes, and foretells its application prospect.

Graphical AbstractAs the acceptor of electron and hydrogen, NAD+ is reduced to from NADH in the catalytic reaction by dehydrogenase,. Then, the reduced coenzyme NADH can be oxidized on electrochemically modified electrodes. Here, the electrodes based on CNT or CNT composite materials for NADH detection were reviewed.Figure optionsDownload as PowerPoint slide