کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1182640 1491636 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid Identification of Multiple Bacteria on a Microfluidic Chip
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Rapid Identification of Multiple Bacteria on a Microfluidic Chip
چکیده انگلیسی

A microfluidic device was developed to integrate sample introduction, bacteria culture and results reading. The identification of multiple bacteria was achieved by combining the spatial resolution of the arrayed bacteria culture chambers and the color resolution benefited from the bacteria specific chromogenic media. A set of 4 common pathogenic bacteria responsible for urinary tract infection was used as a model to test the microfluidic assay. The results showed that the bacteria identification assay was completed in 15 h, with a limit detection (LOD) of bacteria density down to 101 cfu mL−1. Clinical sample testing using the microchip approach showed a coincidence rate of 96.3% as compared with the conventional method. The developed microfluidic approach is simple and rapid, thus hold the potential to serve as a powerful tool for detection of multiple bacteria.

A microfluidic device was developed for rapid identification of multiple bacteria. Various types of bacteria could be simultaneously identified by combining the spatial resolution of arrayed bacteria culture chambers and the color resolution benefited from bacteria specific chromogenic media.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Analytical Chemistry - Volume 42, Issue 6, June 2014, Pages 791–798
نویسندگان
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