کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1183521 | 963248 | 2016 | 7 صفحه PDF | دانلود رایگان |
• Demineralisation of scale was done using 0.75 M HCl for 30 min.
• Ca and P contents of scales markedly decreased after demineralisation.
• HCl removed inorganic matters in the external layer of scales effectively.
Scales of spotted golden goatfish were subjected to non-collagenous protein removal followed by demineralisation with hydrochloric acid at different concentrations (0.25, 0.5, 0.75 and 1 M) for various times (30, 60 and 90 min). The morphology and element composition/distribution of scales from spotted golden goatfish as influenced by demineralisation conditions were determined. The appropriate demineralisation was pertained using 0.75 M hydrochloric acid for 30 min, in which the ash content was 0.62% (dry weight basis). The scales having non-collagenous protein removal with, and without, subsequent demineralisation were analysed for element content using X-ray fluorescence spectrometer. Images of different scales were determined using scanning electron microscopy (SEM) and scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM–EDX). Based on the images, an external layer rich in inorganic elements was removed. Most of Ca and P were eliminated with the coincidental increases in organic substances (C, N and O) after demineralisation. Demineralisation therefore mainly removed the external layer of scales, which facilitated the further extraction of collagen or gelatin.
Journal: Food Chemistry - Volume 197, Part A, 15 April 2016, Pages 814–820