Metabolite extraction for high-throughput FTICR-MS-based metabolomics of grapevine leaves

• An improved metabolite extraction method for DI-FTICR metabolomics was developed.
• The number of identified metabolites from grapevine leaves was maximized.
• The extraction method allowed the extraction of polar and non-polar compounds.
• Identified metabolites covered all major classes found in plants.

In metabolomics there is an ever-growing need for faster and more comprehensive analysis methods to cope with the increase of biological studies. Direct infusion Fourier-transform ion cyclotron-resonance mass spectrometry (DI-FTICR-MS) is used in non-targeted metabolomics to obtain high-resolution snapshots of the metabolic state of a system. In any metabolic profiling study, the establishment of an effective metabolite extraction protocol is paramount. We developed an improved metabolite extraction method, compatible with DI-FTICR-MS-based metabolomics, using grapevine leaves. This extraction protocol allowed the extraction of polar and non-polar compounds, covering all major classes found in plants and increasing metabolome coverage.

Improved and efficient metabolite extraction method for maximizing the number of metabolites identified from a single biological sample, compatible with DI-FTICR-MS-based metabolomics.Figure optionsDownload as PowerPoint slide