Pathway-centric analysis of the DNA damage response to chemotherapeutic agents in two breast cell lines

• Shotgun comparison of two cell lines to chemotherapeutic compounds.
• Development of set of SRM assays covering the six main DNA repair types.
• Comparison of coverage by Shotgun and targeted methods.
• Chemo-resistance can be seen from the DNA repair response.

The response to DNA damage by alkylation and DNA topoisomerase inhibition was studied in two breast cancer cells lines. We present data from both a shotgun and a targeted, pathway-centric approach to highlight the different DNA repair pathway modulation in the cell lines and the correlation with viability and DNA damage assays. This type of focussed profiling may be of utility in rapidly defining non-responders undergoing systemic neoadjuvant therapy.

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