کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11908773 1105219 2019 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Treatment with carbonate buffer decreases antigenicity of nervous necrosis virus (NNV)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Treatment with carbonate buffer decreases antigenicity of nervous necrosis virus (NNV)
چکیده انگلیسی
Antigens are commonly immobilized after suspending them in an alkaline buffer such as carbonate buffer (pH 9.6) for enzyme-linked immunosorbent assay (ELISA). The objective of this study was to determine the effect of 100 mM carbonate buffer (pH 9.6) on antigenicity of nervous necrosis virus (NNV) known to be pathogenic to fish. ELISA values of NNV antigens immobilized using carbonate buffer (pH 9.6) were ≤ 0.23, significantly lower than those by dry immobilization (ELISA values of approximately 1.0). NNV antigenicity was drastically decreased by treatment with carbonate buffer (pH 9.6), but not by treatment with Tris-HCl buffers at pH 8.0 or pH 9.6. Thus, the decrease of NNV antigenicity was due to carbonate/bicarbonate, not pH. NNV infectivity was also decreased after treatment with carbonate buffer (pH 9.6). The anti-NNV serum could recognize conformational structures of NNV surface protrusions related to NNV infection. Therefore, NNV surface protrusions might have been denatured by treatment with carbonate buffer (pH 9.6). Results of this study also demonstrate that coating NNV antigens by buffer immobilization might be far from practical. However, the sensitivity of ELISA detecting NNV antigens can be greatly improved by dry immobilization of antigens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 500, 1 February 2019, Pages 192-195
نویسندگان
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