کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1198689 1493505 2015 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Instrumentation for analytical scale supercritical fluid chromatography
ترجمه فارسی عنوان
ابزار دقیق برای تجزیه و تحلیل مقیاس کروماتوگرافی مایع سوپر کریستالی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Analytical scale supercritical fluid chromatography (SFC) is largely a sub-discipline of high performance liquid chromatography (HPLC), in that most of the hardware and software can be used for either technique. The aspects that separate the 2 techniques stem from the use of carbon dioxide (CO2) as the main component of the mobile phase in SFC. The high compressibility and low viscosity of CO2 mean that pumps, and autosamplers designed for HPLC either need to be modified or an alternate means of dealing with compressibility needs to be found. The inclusion of a back pressure regulator and a high pressure flow cell for any UV-Vis detector are also necessary. Details of the various approaches, problems and solutions are described. Characteristics, such as adiabatic vs. isothermal compressibility, thermal gradients, and refractive index issues are dealt with in detail.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1421, 20 November 2015, Pages 171-183
نویسندگان
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