کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1199291 1493565 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
“Old” metal oxide affinity chromatography as “novel” strategy for specific capture of cis-diol-containing compounds
ترجمه فارسی عنوان
الهه کروماتوگرافی کربن اکسید فلزی به عنوان یک رنل؟ استراتژی برای جذب خاص ترکیبات حاوی سیس دیول
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• The proof-of-concept of expanding MOAC for capturing cis-diol targets was presented.
• ZrO2 or CeO2 could capture cis-diol targets under high pH and release them using acid.
• Endogenous ribonucleosides were purified from urine sample by the MOAC strategy.

The metal oxide affinity chromatography (MOAC) materials have been extensively used for extraction of phosphate compounds in the past decade. Actually, some of these materials also possess adsorption affinity towards cis-diol-containing compounds, which was seldom explored in separation field so far. Here we present the proof-of-concept study to evaluate the feasibility of expanding MOAC for specific capture of cis-diol biomolecules. Benefitting from the high commercialisation of the metal oxide materials, such MOAC strategy possesses several advantages, like synthesis-free, low cost and high expandability. Firstly, the recognition of adenosine against 2′-deoxyadenosine was performed using zirconium oxide and cerium oxide, two typical commercial MOAC materials. The results showed that efficient adsorption and elution could be achieved easily by pH switching from basic to acidic. The isotherm curves demonstrated the adsorption process fitted well with Freundlich isotherm model and was spontaneous at room temperature (ΔG0 < 0) with an exothermic nature (ΔH0 < 0). Afterwards, the highly efficient and selective enrichment of various model cis-diol biomolecules, including ribonucleosides, glycopeptides and glycoproteins, was achieved using this MOAC strategy. Finally, the endogenous ribonucleosides and modified ribonucleosides were successfully purified from human urine sample, which demonstrated the potential application of MOAC materials in the enrichment of target compounds from complex biological samples. Besides the excellent performance of extraction for cis-diol-containing compounds, equally important is that these materials are commercially available with low cost, which makes the MOAC a promising strategy for the study of cis-diol biomolecules in metabolomics and proteomics.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1361, 26 September 2014, Pages 100–107
نویسندگان
, , , , , ,