Application of a new nanocarbonaceous sorbent in electromembrane surrounded solid phase microextraction for analysis of amphetamine and methamphetamine in human urine and whole blood

• A flame-based nanocarbonaceous fiber was prepared on stainless stile.
• The prepared fiber was applied as cathode in electromembrane surrounded solid phase microextraction (EM-SPME).
• EM-SPME technique was applied for extraction of amphetamines from biological fluids.
• The extracted amphetamines were analyzed by GC–FID.
• Quantification of amphetamines was performed in human urine and blood samples.

Application of a new carbon-based sorbent was studied for the first time for extraction and quantification of amphetamine and methamphetamine as model analytes by means of electromembrane surrounded solid phase microextraction (EM-SPME). Since the basis of this microextraction method is adsorption of target analytes on the sorbent surface (after transferring across a supported liquid membrane) in an electrical field, the sorbent, which also performs the electrical potential, should have a conductive nature. On the other hand, using a synthesized fiber is a suitable solution to eliminate the interfering compounds existing in the fiber. To extract the model analytes from acidic sample solution through a thin layer of organic phase and into the aqueous acceptor phase and their final adsorption, 150 V electrical potential was applied for 15 min. Regardless of the high sample cleanup ability of the proposed method, which makes the analysis of complicated biological fluids possible, admissible extraction recoveries (9.0–18.8%) and suitable detection limits (less than 2.0 ng mL−1) were obtained. Repeatability and reproducibility of the method were studied and intra- and inter-assay precisions were in the ranges of 2.0–7.3% and 7.5–12.5%, respectively. Coefficients of determination larger than 0.9964 were achieved by scrutinizing of the linearity up to 500 ng mL−1 and calibration curves were utilized for quantification of analytes of interest in human urine and whole blood samples.