کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1199565 1493536 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Revisiting resolution in hydrodynamic countercurrent chromatography: Tubing bore effect
ترجمه فارسی عنوان
بازبینی رزیدنتی در کروماتوگرافی ضد جرمی هیدرودینامیکی: اثر بخشی لوله
کلمات کلیدی
وضوح، کروماتوگرافی ضد جرمی هیدرودینامیکی، شبیه سازی شده لوله حفاری، بهره وری، طراحی تجهیزات، مطالعه نظری
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A larger bore is beneficial for stationary phase retention.
• A smaller bore produces efficient coil.
• There is an optimal bore tubing that depends on solute polarity.
• Larger bore allows for faster separations.

A major challenge in countercurrent chromatography (CCC), the technique that works with a support-free biphasic liquid system, is to retain the liquid stationary phase inside the CCC column (Sf parameter). Two solutions are commercially available: the hydrostatic CCC columns, also called centrifugal partition chromatographs (CPC), with disks of interconnected channels and rotary seals, and the hydrodynamic CCC columns with bobbins of coiled open tube and no rotary seals. It was demonstrated that the amount of liquid stationary phase retained by a coiled tube was higher with larger bore tubing than with small bore tubes. At constant column volume, small bore tubing will be longer producing more efficiency than larger bore tube that will better retain the liquid stationary phase. Since the resolution equation in CCC is depending on both column efficiency and stationary phase retention ratio, the influence of the tubing bore should be studied. This theoretical work showed that there is an optimum tubing bore size depending on solute partition coefficient and mobile phase flow rate. The interesting result of the theoretical study is that larger tubing bores allow for dramatically reduced experiment durations for all solutes: in reversed phase CCC (polar mobile phase), hydrophobic solutes are usually highly retained. These apolar solutes can be separated by the same coil at high flow rates and reduced Sf with similar retention times as polar solutes separated at smaller flow rates and much higher Sf.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1390, 17 April 2015, Pages 71–77
نویسندگان
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