کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1199964 | 1493572 | 2014 | 10 صفحه PDF | دانلود رایگان |
• New 2D LC-SRM/MS analytical method for prostate specific antigen quantification.
• ng/mL protein biomarkers quantification in plasma without extensive sample preparation before sample injection.
• 2D-LC (RP-HILIC) with an anionic exchange cartridge between both dimensions.
Liquid chromatography (LC) coupled with tandem mass spectrometry (MS–MS) in selected reaction monitoring mode (SRM) has become a widely used technique for the quantification of protein biomarkers in plasma and has already proven to give similar results compared to the conventional immunoassays. To improve the lack of insufficient sensitivity for quantification of low abundance protein, we propose a new two dimensional liquid chromatography (2D-LC-SRM) method for the quantitation of prostate specific antigen (PSA) in human plasma. The method centers on anion exchange cartridge between reversed-phase chromatography and hydrophilic interaction liquid chromatography (HILIC) in an on-line arrangement. The use of the anionic cartridge allows an easier online transfer of the analytes between both dimensions. Moreover, it provides an additional selectivity since the more basic peptides are not retained on this support. This setup has been applied to the quantification of prostate specific antigen (PSA) protein in plasma on a previous generation of mass spectrometer, which enabled a limit of quantification (LOQ) of 1 ng/mL without any upfront immuno-depletion or intense off-line fractionation before the SRM analysis. The obtained LOQ is compatible with the required sensitivity for the clinically relevant plasma-based PSA tests.
Journal: Journal of Chromatography A - Volume 1354, 8 August 2014, Pages 75–84