Equipment and preliminary results for orthogonal pressurized planar electrochromatography

• Overpressured layer chromatography (OPLC).
• Pressurized planar electrochromatography (PPEC).
• Two dimensional orthogonal combination of OPLC and PPEC into simultaneous process.
• Orthogonal pressurized planar electrochromatography (OPPEC).
• Application of OPPEC to preliminary analytical and micro-preparative separations.

We report combination of overpressured layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC) techniques into a single technique in which both OPLC and PPEC processes proceed simultaneously and orthogonally. The separation process with this new technique is performed in adsorbent layer of a chromatographic plate, which is equipped with special sealing margin on its whole periphery and closed under pressure in special chamber. We have named this separation technique as orthogonal pressurized planar electrochromatography (OPPEC). Examples of analytical and micropreparative (continuous) OPPEC separations are demonstrated.