کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1201071 | 1493509 | 2015 | 8 صفحه PDF | دانلود رایگان |
• The prep system allowed to collect highly pure components at milligram level.
• Two configurations were used based on the component concentration.
• No hardware modifications exploiting the two instrument configurations.
• Different stationary phases were exploited to achieve the complete purification.
The present research deals with the multi-collection of the most important sesquiterpene alcohols belonging to sandalwood essential oil, as reported by the international regulations: (Z)-α-santalol, (Z)-α-trans bergamotol, (Z)-β-santalol, epi-(Z)-β-santalol, α-bisabolol, (Z)-lanceol, and (Z)-nuciferol. A versatile multidimensional preparative system, based on the hyphenation of liquid and gas chromatography techniques, was operated in the LC–GC–GC-prep or GC–GC–GC-prep configuration, depending on the concentration to be collected from the sample, without any hardware or software modification. The system was equipped with a silica LC column in combination with polyethylene glycol-poly(5% diphenyl/95% dimethylsiloxane)-medium polarity ionic liquid or β-cyclodextrin based GC stationary phases. The GC–GC–GC-prep configuration was exploited for the collection of four components, by using a conventional split/splitless injector, while the LC–GC–GC-prep approach was applied for three low abundant components (<5%), in order to increase the quantity collected within a single run, by the LC injection of a high sample amount. All target compounds, whose determination is hampered by the unavailability of commercial standards, were collected at milligram levels and with a high degree of purity (>87%).
Journal: Journal of Chromatography A - Volume 1417, 23 October 2015, Pages 96–103