Challenges in the development of bioanalytical liquid chromatography–mass spectrometry method with emphasis on fast analysis

The development of bioanalytical methods has become more and more challenging over the past years due to very demanding requirements in terms of method reliability, sensitivity, speed of analysis and sample throughput. LC–MS/MS has established itself as a method of choice for routine analysis of biological materials. A development of such method consists of several steps including sample preparation and clean-up step, efficient chromatographic separation, sensitive and selective detection of analytes in complex matrices, a choice of convenient data processing and calibration approach and finally method validation. Each of these steps has its own constraints and challenges, which are discussed in detail in this review. Novel and modern approaches in sample preparation, chromatography and detection are especially emphasized. Attention is paid to proper calibration approach and matrix effects that can seriously affect method accuracy and precision.

► The challenges of bioanalytical LC–MS/MS method development are presented.
► Modern and conventional sample preparation techniques are discussed.
► Separation aspect in LC–MS bioanalytical methods.
► Selective and sensitive MS detection in bioanalysis, matrix effects.
► Method validation from the point of view of different authorities.