کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1202185 | 965060 | 2011 | 6 صفحه PDF | دانلود رایگان |
A two-dimensional liquid chromatography system coupled to ion-trap tandem mass spectrometer (2DLC-IT–MS/MS) was employed for the simultaneous quantification of pantoprazole and lansoprazole enantiomers fractions. A restricted access media of bovine serum albumin octyl column (RAM-BSA C8) was used in the first dimension for the exclusion of the humic substances, while a polysaccharide-based chiral column was used in the second dimension for the enantioseparation of both pharmaceuticals. The results described here show good selectivity, extraction efficiency, accuracy, and precision with detection limits of 0.200 and 0.150 μg L−1 for the enatiomers of pantoprazole and lansoprazole respectively, while employing a small amount (1.0 mL) of native water sample per injection. This work reports an innovative assay for monitoring work, studies of biotic and abiotic enantioselective degradation and temporal changes of enantiomeric fractions.
Journal: Journal of Chromatography A - Volume 1218, Issue 20, 20 May 2011, Pages 2865–2870