Evaluation of ionic liquid stationary phases for one dimensional gas chromatography–mass spectrometry and comprehensive two dimensional gas chromatographic analyses of fatty acids in marine biota

Ionic liquid stationary phases were tested for one dimensional gas chromatography–mass spectrometry (GC–MS) and comprehensive two dimensional gas chromatography (GC × GC) of fatty acid methyl esters from algae. In comparison with polyethylene glycol and cyanopropyl substituted polar stationary phases, ionic liquid stationary phases SLB-IL 82 and SLB-IL 100 showed comparable resolution, but lower column bleeding with MS detection, resulting in better sensitivity. The selectivity and polarity of the ionic liquid phases are similar to a highly polar biscyanopropyl-silicone phase (e.g. HP-88). In GC × GC, using an apolar polydimethyl siloxane × polar ionic liquid column combination, an excellent group-type separation of fatty acids with different carbon numbers and number of unsaturations was obtained, providing information that is complementary to GC–MS identification.