کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1202406 | 1493674 | 2012 | 4 صفحه PDF | دانلود رایگان |
A new disposable adsorbent material for fast anion-exchange capture of nano-complexes without prefiltering, clarification or pre-processing of samples was developed based on plastic microcapillary films (MCFs). An MCF containing 19 parallel microcapillaries, each with a mean internal diameter of 142 ± 10 μm, was prepared using a melt extrusion process from an ethylene–vinyl alcohol copolymer (EVOH). The MCF internal surfaces were functionalised using branched chain chemistries to attach quaternary amine groups producing an anion-exchange adsorbent. The purification of nano-complexes using this newly fabricated MCF–EVOH-Q was successfully demonstrated with the capture of lentivirus from pre-filtered culture harvest. This 5 m chromatographic substrate was found to bind and elute ∼40% of bound lentivirus or 2.5 × 106 infectious units (ifu). The unique properties of this chromatographic substrate that allow the passage of large particulates was further demonstrated with the capture of lentiviral particles from unfiltered un-processed culture media containing cells and cell debris. Using this approach, 56% or 1 × 107 ifu of captured lentivirus was eluted. A device based on this new material might be used at an early stage in clinical lentiviral production to harvest lentiviral particles, directly from bioreactors.
► New disposable adsorbent material developed for fast anion-exchange capture of nano-complexes.
► No sample prefiltering, clarification or pre-processing required.
► Consists of melt extruded plastic films containing 19 parallel microcapillaries.
► Capture of 1 × 107 infectious units of lentivirus (∼clinical trial does) from un-processed sample.
► Might be used to harvest lentiviral particles directly from bioreactors in clinical production.
Journal: Journal of Chromatography A - Volume 1251, 17 August 2012, Pages 236–239