کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1202598 965076 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chemical composition of bioactive pressurized extracts of Romanian aromatic plants
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Chemical composition of bioactive pressurized extracts of Romanian aromatic plants
چکیده انگلیسی

In this contribution, pressurized liquid extraction (PLE) has been employed to isolate bioactive compounds from three native Romanian plants, oregano (Origanum vulgare), tarragon (Artemisia dracunculus) and wild thyme (Thymus serpyllum). Different PLE conditions have been tested including extraction with water, ethanol and their mixtures in a wide range of extraction temperatures (50–200 °C), and the antioxidant capacity of the extracts was measured using different assays (DPPH radical scavenging, TEAC assay and Folin–Ciocalteau assay to measure total phenols). Moreover, a complete chemical characterization by using LC–MS/MS was carried out to be able to correlate the bioactivity with the particular chemical composition of each extract and plant. The use of PLE with water as a solvent at the highest temperature tested (200 °C) always provided the highest extraction yields for the three studied plants, being maximum for oregano (>60%). Besides, oregano's pressurized water extracts at lower temperatures (50 °C) presented the highest content on total phenols (184.9 mg gallic acid/g extract) and the best antioxidant activities (EC50 6.98 μg/ml). In general, oregano extracts were the most active, followed by wild thyme extracts. The antioxidant capacity measured by DPPH assay was highly correlated with the amount of total phenols. Moreover, the use of a LC–MS/MS method allowed the identification of 30 different phenolic compounds in the different extracts, including phenolic acids, flavones, flavanones and flavonols, which have an important influence on the total antioxidant capacity of the different extracts.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 30, 29 July 2011, Pages 4918–4927
نویسندگان
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