کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1202768 1493558 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Use of individual retention modeling for gradient optimization in hydrophilic interaction chromatography: Separation of nucleobases and nucleosides
ترجمه فارسی عنوان
استفاده از مدل سازی احتباس فردی برای بهینه سازی شیب در کروماتوگرافی متقابل هیدروفیل: جداسازی هسته های هسته ای و نوکلئوزید ها
کلمات کلیدی
توسعه روش های کامپیوتری، مدل سازی احتباس فردی، هسته های پایه، نوکلئوزید، هیلیک
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• HILIC gradient retention modeling.
• Gradient optimization via individual retention modeling.
• Extended Neue and Kuss model for coupled column system.
• Separation of twelve nucleobases and nucleosides in less than 7 min.

In this study, the separation of twelve nucleobases and nucleosides was optimized via chromatogram simulation (i.e., prediction of individual retention times and estimation of the peak widths) with the use of an empirical (reversed-phase) non-linear model proposed by Neue and Kuss. Retention time prediction errors of less than 2% were observed for all compounds on different stationary phases. As a single HILIC column could not resolve all peaks, the modeling was extended to coupled-column systems (with different stationary phase chemistries) to increase the separation efficiency and selectivity. The analytical expressions for the gradient retention factor on a coupled column system were derived and accurate retention time predictions were obtained (<2% prediction errors in general). The optimized gradient (predicted by the optimization software) included coupling of an amide and an pentahydroxy functionalized silica stationary phases with a gradient profile from 95 to 85%ACN in 6 min and resulted in almost baseline separation of the twelve nucleobases and nucleosides in less than 7 min. The final separation was obtained in less than 4 h of instrument time (including equilibration times) and was fully obtained via computer-based optimization. As such, this study provides an example of a case where individual retention modeling can be used as a way to optimize the gradient conditions in the HILIC mode using a non-linear model such as the Neue and Kuss model.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1368, 14 November 2014, Pages 125–131
نویسندگان
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