Estimation of the uncertainty associated with the results based on the validation of chromatographic analysis procedures: Application to the determination of chlorides by high performance liquid chromatography and of fatty acids by high resolution gas chr

This article presents a model to calculate the uncertainty associated with an analytical result based on the validation of the analysis procedure. This calculation model is proposed as an alternative to commonly used bottom-up and top-down methods. This proposal is very advantageous as the validation of the procedures and the estimation of the uncertainty of the measurement are part of the technical requirements needed in order to obtain the ISO 17025:2005 accreditation. This model has been applied to the determination of chloride by liquid chromatography in lixiviates and in the determination of palmitic acid and stearic acid by gas chromatography in magnesium stearate samples.

► The uncertainty characterizes the dispersion of the values of a measure.
► We present a model to estimate it based on validation of analytical procedures.
► This fact is a great advantage of time and effort compared to other models.
► This model has been applied to determinate chlorides by HPLC and fatty acids by GC.
► This model allows to identify all sources of error in the calculation of uncertainty.