Combination of corona discharge ion mobility spectrometry with a novel reagent gas and two immiscible organic solvent liquid-liquid-liquid microextraction for analysis of clomipramine in biological samples

► A method based on two immiscible organic solvent liquid-liquid-liquid microextraction and ion mobility spectroscopy is reported. ► Effect of formic, acetic and propionic acid as the reagent gas (dopant) on the corona discharge ion mobility signal was investigated. ► Clomipramine was successfully determined in human urine and plasma by the method. ► The method shows relatively low detection limit, good precision, high recovery and efficient sample clean-up.