کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1203365 965110 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of deoxynivalenol, T-2 and HT-2 toxins in a bread model food by liquid chromatography–high resolution-Orbitrap-mass spectrometry equipped with a high-energy collision dissociation cell
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of deoxynivalenol, T-2 and HT-2 toxins in a bread model food by liquid chromatography–high resolution-Orbitrap-mass spectrometry equipped with a high-energy collision dissociation cell
چکیده انگلیسی

A sensitive and accurate method employing a single stage high resolution mass spectrometer equipped with a high-energy collision-dissociation cell (HCD) for the simultaneous determination of deoxynivalenol (DON), T-2 toxin (T-2) and HT-2 toxin (HT-2) in a processed bread model food has been developed. Two sample pre-treatment routes for the extraction of these mycotoxins were investigated, based on Mycosep® column clean up or QuEChERS-like procedure, respectively. The former approach suffered less from matrix effects and allowed to achieve in bread samples LODs of 7, 12 and 17 ng/g for T-2, HT-2 and DON, respectively, with 0.5 ppm mass accuracy. Two acquisition modes, full scan MS and all ion fragmentation, exploiting the fragmentation features offered by an HCD chamber and integrated within the Orbitrap analyser, were compared for quantitative purposes. The method was applied to investigate the degradation of these mycotoxins during bread processing using a bread model food. Most T-2 hydrolyzed to HT-2 during dough preparation, and about 20–30% of HT-2 and DON was degraded during bread baking.


► Quantitative method for DON, T2 and HT2 detection by single-stage Orbitrap MS.
► Full MS and HCD-MS modes proved to be suitable for quantification purposes.
► Two sample pretreatment routes for mycotoxins extraction from bread were compared.
► Mycosep-based approach was the best in terms of sensitivity and matrix-effect.
► Extent of mycotoxin degradation during bread-baking assessed by LC-HR-MS method.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 48, 2 December 2011, Pages 8646–8654
نویسندگان
, , ,