کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1203490 | 965115 | 2011 | 9 صفحه PDF | دانلود رایگان |
A family of linear hexamer peptide ligands HWRGWV, HYFKFD and HFRRHL, initially identified for their affinity to the Fc portion of human immunoglobulin G (hIgG), also have potential for use in the purification of human immunoglobulins A (hIgA) and M (hIgM). HWRGWV demonstrated the strongest binding affinity to hIgM, followed by hIgA and hIgG respectively. The effects of N-terminal acetylation of the peptide, as well as elution buffer pH, on the chromatographic elution of human IgG, IgA and IgM from HWRGWV resins at various peptide densities (0.04–0.55 meq/g) were investigated. Over 80% recovery and 90% purity were achieved for human IgG and IgA isolation from complete minimum essential medium (cMEM) using HWRGWV resin at optimum peptide densities. For human IgM, 75.7% recovery and 86.0% purity were achieved by using HWRGWV at a low peptide density of 0.04 meq/g. Although HYFKFD and HFRRHL exhibited their ability for isolation of human IgG, IgA and IgM from cMEM as well, HWRGWV is the best option among them for large-scale purification of human IgG, IgA and IgM based on conditions tested.
► Peptide ligands were used to purify IgM and IgA from complex media.
► Ligand displayed strongest binding affinity to hIgM, followed by hIgA and hIgG.
► Recoveries were higher at high ligand density, but purities decreased.
► Over 80% recovery and 90% purity achieved for IgA from culture medium using HWRGWV.
► For IgM, 75.7% recovery and 86.0% purity achieved at a peptide density of 0.04 meq/g.
Journal: Journal of Chromatography A - Volume 1218, Issue 46, 18 November 2011, Pages 8344–8352