کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1203641 965120 2011 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Screening, identification and quantification of glucosinolates in black radish (Raphanus sativus L. niger) based dietary supplements using liquid chromatography coupled with a photodiode array and liquid chromatography - mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Screening, identification and quantification of glucosinolates in black radish (Raphanus sativus L. niger) based dietary supplements using liquid chromatography coupled with a photodiode array and liquid chromatography - mass spectrometry
چکیده انگلیسی

The glucosinolate profile of black radish (Raphanus sativus L. niger) based dietary supplements has been investigated by HPLC-PDA, LC-ESI-MS/MS and LC-APCI-MS/MS systems. Optimization of the MS/MS parameters and LC conditions was performed using sinigrin reference standard and rapeseed certified reference material (BC190) respectively. An LC-ESI-MS/MS system was used to detect (screen) and identify the naturally occurring intact glucosinolates (GLs). The intact GLs identified were then desulfated and quantified on an HPLC-PDA system as desulfo-glucosinolates (DS-GLs). Prior to quantification, the DS-GLs were identified using an APCI-MS/MS. The HPLC-PDA method performance criteria were evaluated using glucotropaeolin potassium salt. The validated method was applied for the analysis of six dietary supplements. In total, six glucosinolates were identified and quantified in the dietary supplements; glucoraphasatin (0.2–0.48 mg/g), glucosisaustricin (0.37–0.91 mg/g), glucoraphenin (0.84–1.27 mg/g), glucoputrajivin (0.14–0.28 mg/g), glucosisymbrin (0.70–0.99 mg/g) and gluconasturtiin (0.06–0.12 mg/g). Glucoraphenin was the most abundant glucosinolate in all samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 28, 15 July 2011, Pages 4395–4405
نویسندگان
, , , , , , , , ,