کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1203822 | 965127 | 2011 | 6 صفحه PDF | دانلود رایگان |
Furaneol is an important aroma compound. It is very difficult to extract furaneol from food matrices and separate it on a gas chromatography column due to its high polarity and instability. A new quantitative method was developed to quantify furaneol in aqueous samples by the use of derivatization/solid phase microextraction (SPME) coupled with gas chromatography/mass spectrometry (GC/MS). The derivatization was carried out by reacting pentafluorobenzyl bromide with furaneol in basic solutions at elevated temperatures. The derivative was stable and less polar so that SPME-GC/MS could be applied for extraction, separation and detection. The automated analytical method had a limit of detection (LOD) of 0.5 ng mL−1, a limit of quantification (LOQ) of 2 ng mL−1, a repeatability of 9.5%, and a linear range from 2 to 500 ng mL−1. The method was applied to analyze fruit samples. And it was found that the concentrations of furaneol in tomato ranged from 95 to 173 μg kg−1, in strawberries ranged from 1663 to 4852 μg kg−1. The results were verified with a LC procedure. To facilitate analytical method development, some physico-chemical parameters for furaneol were determined in this work. Its solubility in water was determined as 0.315 g mL−1 (25 °C). Its Log D in water and Log P in 0.1 M phosphate buffer were −0.133 and 0.95 (20 °C), respectively. Its pKa was 8.56 (20 °C).
Journal: Journal of Chromatography A - Volume 1218, Issue 38, 23 September 2011, Pages 6817–6822