Separation of piracetam derivatives on polysaccharide-based chiral stationary phases

High-performance liquid chromatography was used for the enantiomeric separation of two chiral piracetam derivatives. The suitability of six commercially available polysaccharide-based chiral stationary phases (CSPs) under normal phase mode for direct enantioseparation has been investigated. The influence of the CSPs as well the nature and content of an alcoholic modifier in the mobile phase on separation and elution order was studied. It was established that CSP Lux Amylose-2 shows high chiral recognition ability towards 4-phenylsubstituted piracetam derivatives.

► Enantioseparation of two chiral drugs was examined.
► High chiral recognition ability of column Lux Amylose-2 was observed.
► The influence of an alcoholic modifier on enantioseparation was studied.