کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1205220 | 965187 | 2010 | 7 صفحه PDF | دانلود رایگان |
Enantioseparation of 1,1′-bi-2-naphthol (BINOL) was performed on a polysaccharide-based chiral stationary phase, Chiralcel OD-H, under normal-phase mode. The effects of polar modifier in the mobile phase on the retention, enantioseparation and elution order were investigated in detail. Solvent-induced reversal of elution order for BINOL was observed. When linear alcohols were adopted, R-BINOL was always eluted first. S-BINOL was eluted first when 2-propanol was used as a polar modifier. Enantioseparation could not be obtained when sec-butyl alcohol or tert-butyl alcohol was used as a polar modifier. When isoamyl alcohol or cyclohexanol was used as a polar modifier, favorable enantioseparation was obtained as with 1-pentanol or 1-hexanol; also, R-BINOL was the first-eluted enantiomer. It is worth emphasizing that significantly better enantioseparation was obtained when higher alcohols were used as polar modifier of the mobile phase. A nonlinear characteristic for the ln α against 1/T plots was universally observed in this study though the ln k against 1/T plots exhibited a linear feature. Associated with the obtained thermodynamic parameters, some interesting inferences about chiral recognition mechanism were proposed.
Journal: Journal of Chromatography A - Volume 1217, Issue 26, 25 June 2010, Pages 4278–4284