کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1205411 965195 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reaction of fluorogenic reagents with proteins: II: Capillary electrophoresis and laser-induced fluorescence properties of proteins labeled with Chromeo P465
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Reaction of fluorogenic reagents with proteins: II: Capillary electrophoresis and laser-induced fluorescence properties of proteins labeled with Chromeo P465
چکیده انگلیسی

The fluorogenic reagent Chromeo P465 is considered for the analysis of proteins by capillary electrophoresis with laser-induced fluorescence detection. The reagent was first used to label α-lactalbumin; the product was analyzed by capillary zone electrophoresis in a sub-micellar sodium dodecyl sulfate (SDS) buffer. The product generated a set of equally spaced but poorly resolved peaks that formed a broad envelope with a net mobility of 4 × 10−4 cm2 V−1 s−1. The components of the envelope were presumably protein that had reacted with different numbers of labels. The mobility of these components decreased by roughly 1% with the addition of each label. The signal increased linearly from 1.0 nM to 100 nM α-lactalbumin (r2 = 0.99), with a 3σ detection limit of 70 pM. We then considered the separation of a mixture of ovalbumin, α-chymotrypsinogen A, and α-lactalbumin labeled with Chromeo P465; unfortunately, baseline resolution was not achieved with a borax/SDS buffer. Better resolution was achieved with N-cyclohexyl-2-aminoethanesulfonic acid/Tris/SDS/dextran capillary sieving electrophoresis; however, dye interactions with this buffer system produced a less than ideal blank.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1194, Issue 2, 20 June 2008, Pages 249–252
نویسندگان
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