کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1205412 965195 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reaction of fluorogenic reagents with proteins: III. Spectroscopic and electrophoretic behavior of proteins labeled with Chromeo P503
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Reaction of fluorogenic reagents with proteins: III. Spectroscopic and electrophoretic behavior of proteins labeled with Chromeo P503
چکیده انگلیسی

The spectroscopic and electrophoretic properties of proteins labeled with Chromeo P503 were investigated. Its photobleaching characteristics were determined by continually infusing Chromeo P503-labeled α-lactalbumin into a sheath-flow cuvette and monitored fluorescence as a function of laser power. The labeled protein is relatively photo-labile with an optimum excitation power of about 2 mW. The unreacted reagent is weakly fluorescent but present at much higher concentration than the labeled protein. The unreacted reagent undergoes photobleaching at a laser power more than an order of magnitude higher than the labeled protein. One-dimensional capillary electrophoresis analysis of Chromeo P503-labeled α-lactalbumin produced concentration detection limits (3σ) of 12 pM and mass detection limits of 0.7 zmol, but with modest theoretical plate counts of 17,000. The reagent was employed for the two-dimensional capillary electrophoresis analysis of a homogenate prepared from a Barrett's esophagus cell line; the separation quality is similar to that produced by 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ), a more commonly used reagent.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1194, Issue 2, 20 June 2008, Pages 253–256
نویسندگان
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