کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1205626 | 965202 | 2009 | 9 صفحه PDF | دانلود رایگان |
Simulated moving bed (SMB) chromatography combines high productivity and high purities with reduced buffer consumption. We have developed a laboratory scale single column SMB (SC-SMB) unit with all four separation zones in one column. Distributors embedded within the chromatographic medium allow introduction and withdrawal of liquid between the zones. This single column unit exhibits homogenous packing in all zones, reduced headspace, less complex tubing, fewer valves, and almost undisturbed plug flow between the separation zones. The separation performance of the column was investigated with two different binary model mixtures. Furthermore, the SC-SMB unit is operated with a modified AKTA Explorer workstation, which has been specifically developed for the handling of biological fluids.
Journal: Journal of Chromatography A - Volume 1216, Issue 50, 11 December 2009, Pages 8778–8786