کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1206069 | 1493731 | 2007 | 14 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins Contribution of solid-phase hexapeptide ligand libraries to the repertoire of human bile proteins](/preview/png/1206069.png)
Proteins in bile may have important physiological functions and serve as disease biomarkers. Here, the protein composition of human gallbladder bile was analyzed using a recently described chromatography-like technology capable to enhance the signal of low-abundance species. First, proteins present in bile fluid were treated with immobilized peptide ligand libraries to concentrate dilute and very dilute species while concomitantly diluting the high-abundance proteins. The analysis of resulting protein mixture was then performed using LC–MS/MS after having classically separated proteins by a mini preparative gel electrophoresis. Overall 222 gene products were found; 143 of them were not reported before in proteomics studies. Ligand libraries by themselves contributed to find 81 new gene products distributed throughout different categories. The described chromatographic approach provides a significant contribution to the bile protein repertoire and opens new perspectives for the discovery of markers for specific biliary tract diseases.
Journal: Journal of Chromatography A - Volume 1176, Issues 1–2, 28 December 2007, Pages 192–205