کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1206396 | 965232 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Influence of ligand density on antibody binding capacity of cation-exchange adsorbents
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Adsorption properties of a set of polymethacrylate-based cation exchangers designed for purification of monoclonal antibodies were investigated. The materials differed significantly in the density of sulphoisobutyl ligand groups. The ligand density had a pronounced effect on the static adsorption capacity of a polyclonal human immunoglobulin G. An optimal ligand density was observed at any pH and NaCl concentration tested when sharp optima were observed at low pH and ionic strength values. This was caused by effective clogging of pore mouth at high ligand densities. An anomalous effect of ionic strength was observed for the adsorbents with the high ligand density when the adsorption capacity increased with the addition of NaCl at low pH.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1216, Issue 25, 19 June 2009, Pages 5039–5044
Journal: Journal of Chromatography A - Volume 1216, Issue 25, 19 June 2009, Pages 5039–5044
نویسندگان
Katarzyna Wrzosek, Michal Gramblička, Milan Polakovič,