کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1206542 965238 2008 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Multi-compound electrophoretic assays for tyramine oxidase with a UV area detector imaging multiple windows on a looped capillary
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Multi-compound electrophoretic assays for tyramine oxidase with a UV area detector imaging multiple windows on a looped capillary
چکیده انگلیسی

Active pixel sensor UV area imaging and capacitively coupled contactless conductivity detection have been applied in an electrophoretically mediated microanalysis (EMMA) assay for substrate specificity of tyramine oxidase (Arthrobacter sp.). Use of the UV area imaging detector to monitor four windows in a capillary with three loops provided intrinsic self-referencing for all species and identified tyramine and 2-phenethylamine as the only reactive components in a multi-compound mixture. Continuous engagement EMMA experiments showed significant benefits by comparison with plug–plug EMMA, improving sensitivity by extending enzyme–substrate interaction times and allowing measurement of time-dependent reaction in the substrate zones passing the four windows.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1206, Issue 1, 3 October 2008, Pages 52–63
نویسندگان
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