کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1206650 965242 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel collagenase-assisted extraction of active pharmaceutical ingredients from gelatin products for quantitative analysis by high performance liquid chromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A novel collagenase-assisted extraction of active pharmaceutical ingredients from gelatin products for quantitative analysis by high performance liquid chromatography
چکیده انگلیسی

High performance liquid chromatography (HPLC) is frequently used for quantifying drugs in gelatin capsules. Gelatin has a strong UV absorption that often overlaps with drug absorbances. Therefore, the gelatin capsules must be removed manually before analysis. This study describes a novel extraction method named collagenase-assisted extraction (CAE), which uses collagenase to degrade gelatin into fragments with reduced hydrophobicity, allowing gelatin-related peaks to elute immediately, eliminating interference with the drug peak and enabling use of whole gelatin capsules during drug quantification. Use of CAE eliminates powder loss when opening a gelatin capsule, allows extraction of drugs from the capsule, reduces analytical time, and extends HPLC column life.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1216, Issue 20, 15 May 2009, Pages 4524–4528
نویسندگان
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