کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1206672 | 1493717 | 2008 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: High-performance liquid chromatography analysis methods developed for quantifying enzymatic esterification of flavonoids in ionic liquids High-performance liquid chromatography analysis methods developed for quantifying enzymatic esterification of flavonoids in ionic liquids](/preview/png/1206672.png)
Methods using reversed-phase high-performance liquid chromatography (RP-HPLC) with ELSD were investigated to quantify enzymatic reactions of flavonoids with fatty acids in the presence of diverse room temperature ionic liquids (RTILs). A buffered salt (preferably triethylamine–acetate) was found essential for separation of flavonoids from strongly polar RTILs, whereby RTILs were generally visible as two major peaks identified based on an ion-pairing/exchanging hypothesis. C8 and C12 stationary phases were optimal while mobile phase pH (3–7) had only a minor influence on separation. The method developed was successfully applied for primary screening of RTILs (>20), with in depth evaluation of substrates in 10 RTILs, for their evaluation as reaction media.
Journal: Journal of Chromatography A - Volumes 1198–1199, 11 July 2008, Pages 107–114