کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1206761 965245 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of capillary cross-section geometry and size on the separation of proteins in gradient mode using monolithic poly(butyl methacrylate-co-ethylene dimethacrylate) columns
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Effect of capillary cross-section geometry and size on the separation of proteins in gradient mode using monolithic poly(butyl methacrylate-co-ethylene dimethacrylate) columns
چکیده انگلیسی

Porous polymer monoliths have been prepared in capillaries with circular or square cross-sections and lateral dimensions of 50, 75, 100 μm as well as in a rectangular 38 μm × 95 μm capillary. These capillaries have been used to determine the effect of the size and shape of their cross-section on the porous and hydrodynamic properties of poly(butyl methacrylate-co-ethylene dimethacrylate) monoliths. The capillaries were studied by scanning electron microscopy and evaluated for their permeability to flow and their performance in the liquid chromatographic separation of a protein mixture comprising ribonuclease A, cytochrome c, myoglobin, and ovalbumin using a linear gradient of acetonitrile in the mobile phase. No differences resulting from channel geometry were found for the various capillary columns. These results demonstrate that standard capillaries with circular geometry are a good and affordable alternative conduit for modeling the processes carried out in microfluidic chips with a variety of geometries.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1216, Issue 12, 20 March 2009, Pages 2355–2361
نویسندگان
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