کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1207183 1493747 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of a novel modification on IgG2 light chain: Evidence for the presence of O-linked mannosylation
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Characterization of a novel modification on IgG2 light chain: Evidence for the presence of O-linked mannosylation
چکیده انگلیسی

This paper describes the analysis of a novel modification identified on the light chain of a recombinant IgG2 antibody. This modification, a +162 Da adduct, suggestive of a single hexose addition, was observed by mass analysis of the reduced molecule. The modification was located on residue serine 66 of the light chain by investigation with LC–MS peptide mapping, mass spectrometry and N-terminal sequencing techniques. Location of the adduct on serine pointed the investigation toward O-linked glycosylation. Identification of the hexose residue was deduced from its elimination by action of α-mannosidase, providing evidence for the presence of an O-mannosylated light chain. This type of modification in the glycosylation profile of antibodies, to our knowledge, has not been reported for human IgG molecules.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1156, Issues 1–2, 13 July 2007, Pages 183–187
نویسندگان
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