کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1207194 1493747 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A sensitive liquid chromatographic method for the analysis of isovaleric and valeric acids in urine as fluorescent derivatives
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A sensitive liquid chromatographic method for the analysis of isovaleric and valeric acids in urine as fluorescent derivatives
چکیده انگلیسی

A simple and sensitive isocratic liquid chromatographic method was developed for the analysis of isovaleric and valeric acids in human urine as biomarkers in metabolic acidosis. The method is based on the derivatization of isovaleric and valeric acids with a fluorescent reagent 2-(2-naphthoxy)ethyl-2-(piperidino)ethanesulfonate for labeling the analytes with the naphthoxy fluorophore. The resulting fluorescent derivatives of isovaleric and valeric acids were separated on a phenyl-hexyl column, using a mixed solvent of methanol–water–tetrahydrofuran (55:31:14, v/v) as the mobile phase. The separated derivatives were monitored with a fluorimetric detector (excitation at 225 nm and emission at 360 nm). The linear range of the method for the determination of isovaleric acid or valeric acid derivative was over 0.2 ∼ 8.0 μM. The detection limit (signal to noise ratio = 3 with 10 μl injected) of isovaleric acid or valeric acid was about 0.04 μM. Application of the method to the analysis of isovaleric acid in the urine of a patient with isovaleric acidemia proved feasible.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1156, Issues 1–2, 13 July 2007, Pages 259–263
نویسندگان
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