کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1209390 | 1493755 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a new diagnostic method for galactosemia by high-performance anion-exchange chromatography with pulsed amperometric detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We developed a new non-derivatization analytical method for the determination of galactose in the diagnosis of galactosemia by high-performance anion-exchange chromatography (HPAEC)-pulsed amperometric detection (PAD). With an anion-exchange column, the analytes were separated efficiently using 3 mM NaOH containing 1 mM NaOAc, and 200 mM NaOH was added for post-column reagent. The limit of detection (S/N = 3) and limit of quantification (S/N = 10) for galactose were 25 ng/mL and 83 ng/mL, respectively. Linear dynamic range was from 4.67 mg/dL to 53.46 mg/dL (r2 = 0.9999). The mean recovery of galactose for intra-, inter-day assays were found to be of satisfactory results (98.14–101.42%).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1140, Issues 1–2, 26 January 2007, Pages 157–162
Journal: Journal of Chromatography A - Volume 1140, Issues 1–2, 26 January 2007, Pages 157–162
نویسندگان
Ji-Seon Jeong, Hye-Ran Yoon, Seon-Pyo Hong,