کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1209453 | 965343 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Apolar chromatography on Sephadex LH-20 combined with high-speed counter-current chromatography: High yield strategy for structurally closely related analytes—Destruxin derivatives from Metarhizium anisopliae as a case study Apolar chromatography on Sephadex LH-20 combined with high-speed counter-current chromatography: High yield strategy for structurally closely related analytes—Destruxin derivatives from Metarhizium anisopliae as a case study](/preview/png/1209453.png)
A novel high yield isolation procedure for lipophilic cyclic peptide derivatives is presented. Destruxin (dtx) A, B, D, E, and E-diol retrieval from Metarhizium anisopliae culture broth was achieved with a three-step purification protocol. After liquid–liquid extraction column chromatography over Sephadex LH-20 served as enrichment step. High-speed counter-current chromatography (HSCCC) was used for the final purification. Within the first chromatographic step dtx D and dtx E-diol were separated in purities exceeding 90%. The separation of dtx A, B, and E was achieved from an enriched Sephadex LH-20 fraction by a HSCCC protocol using light petroleum–ethyl acetate–methanol–water = 2:5:2:5 (v/v) as eluent system. These derivatives were obtained in purities above 98% and total yields exceeding 40%.
Journal: Journal of Chromatography A - Volume 1117, Issue 1, 2 June 2006, Pages 67–73