کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1209491 | 1493757 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Simultaneous determination of 11 characteristic components in three species of Curcuma rhizomes using pressurized liquid extraction and high-performance liquid chromatography Simultaneous determination of 11 characteristic components in three species of Curcuma rhizomes using pressurized liquid extraction and high-performance liquid chromatography](/preview/png/1209491.png)
A high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) and pressurized liquid extraction was developed for simultaneous quantitative determination of 11 characteristic compounds, including curcumenone, curcumenol, neocurdione, curdione, isocurcumenol, furanodienone, curcumol, germacrone, curzerene, furanodiene and β-elemene, in rhizomes of three species of Curcuma. The analysis was performed on an ODS C18 column. The mobile phase consisted of (A) water and (B) acetonitrile using a gradient elution. The peaks were monitored at both 214 nm and 256 nm. All calibration curves showed good linearity (r2 > 0.9996) within test ranges. This method showed good repeatability for the quantification of these eleven components in three species Curcuma rhizomes with intra- and inter-day variations of less than 1.57% and 1.98%, respectively. The validated method was successfully applied to quantify 11 investigated components in eighteen samples of three species of Curcuma, which is helpful to control their quality.
Journal: Journal of Chromatography A - Volume 1134, Issues 1–2, 17 November 2006, Pages 226–231