کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1210330 | 1493777 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Looking inside the pores of a chromatographic column
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The dependence of void volume and pore volume on mobile phase composition was studied on a reversed phase column based on poly(divinyl benzene) in mobile phases consisting of 100% tetrahydrofuran (THF) and 40-90% THF-water. Void volumes V0 are obtained from a series of polymers with different molar mass by extrapolation of elution volume to M = 0, the interstitial volume Vi from the elution volume of polymers with a sufficiently high molar mass. The pore volume Vp is obtained as the difference (V0 â Vi). While Vi does not depend on mobile phase composition, a dramatic decrease of the pore volume is observed, when the mobile phase is changed from THF to THF-water. This effect of the mobile phase composition on the pore volume is observed using PEG and PPG as test macromolecules, regardless the retention mechanism: at 40-50% THF both PEG (in exclusion mode) and PPG (in adsorption mode) experience the same reduced pore volume, which is considerably lower than that in 100% THF.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1104, Issues 1â2, 3 February 2006, Pages 238-244
Journal: Journal of Chromatography A - Volume 1104, Issues 1â2, 3 February 2006, Pages 238-244
نویسندگان
Bernd Trathnigg, Martin Veronik, Alexei Gorbunov,