کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1210533 965377 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a microcapillary column for detecting targeted messenger RNA molecules
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of a microcapillary column for detecting targeted messenger RNA molecules
چکیده انگلیسی
A capillary column in a rapid-flow system has been developed for detecting targeted messenger RNA (mRNA) molecules. The column has a structure made of two beds-one bed of porous microbeads and one bed of microbeads with a polythymidine base sequence. The targeted eukaryotic mRNA molecules are detected by two-step hybridization (sandwich hybridization) composed of polyadenosine selection of mRNA molecules and formation of a probe-target (targeted mRNA) hybrid. The sandwich hybridization, which is accomplished within 1 h, was tested using synthetic polydeoxynucleotides. Ten picomoles of the targeted polydeoxynucleotide were detected.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1109, Issue 2, 24 March 2006, Pages 122-126
نویسندگان
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