کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1211011 965453 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid quantification of total genomic DNA methylation degree by short-end injection capillary zone electrophoresis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Rapid quantification of total genomic DNA methylation degree by short-end injection capillary zone electrophoresis
چکیده انگلیسی

We propose a new capillary zone electrophoresis method applying short-end injection technique for the fast evaluation of methylcystosine/total cytosine ratio after acidic DNA hydrolysis. By short-end injection and by using a 100 mmol/l Tris solution titrated with 1 mol/l phosphoric acid to pH 3.75 as background electrolyte, cytosine and methylcytosine were separated with a good resolution in less than 1.5 min. Stepwise multiple linear regression with DNA methylation degree as the dependent variable and age, cysteine, homocysteine and methionine as independent variables, showed a negative association with age and that total cysteine is the most important determinant of DNA methylation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1185, Issue 1, 21 March 2008, Pages 145–150
نویسندگان
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