کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1211369 | 1493760 | 2006 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: High-performance liquid chromatography determination of phenyllactic acid in MRS broth High-performance liquid chromatography determination of phenyllactic acid in MRS broth](/preview/png/1211369.png)
Phenyllactic acid (PLA) is an organic acid produced by some strains of lactic acid bacteria (LAB) and concentrations higher than 7.5 mg/ml inhibit growth of moulds and yeasts. Since PLA can be used to select LAB, a rapid, simple and cheap method for its determination is desirable. Typical methods for its analysis in broth are time-consuming, analytically complicated, and have poor recoveries. Herein we propose a simple and rapid method that does not require extraction, but only microfiltration of broth before injection in HPLC. The improved chromatographic conditions allow separation and quantification of PLA with a recovery of 98.7%. The method is highly reproducible with an intraday repeatability of the total peak area of 2.00%, while an interday repeatability of 2.69%.
Journal: Journal of Chromatography A - Volume 1131, Issues 1–2, 27 October 2006, Pages 281–284